Combination Strategy of Two Hydrogels Loaded with Prussian Blue Nanoparticles and Calcium Peroxide Separately for Diabetic Wound Repair

Oxidative stress and excessive hypoxia are two key factors hindering diabetic wound healing. Prussian blue nanoparticles (PBNPs) and calcium peroxide (CPO) possess the characteristics of scavenging reactive oxygen species (ROS) and chemically generating oxygen respectively, and both can be used as biomedical materials. However, when the two substances coexist in the aqueous media, Prussian blue undergoes decomposition. In this study, gelatin/carboxymethyl chitosan @PBNP hydrogel and gelatin/carboxymethyl chitosan@CPO hydrogel were prepared separately and combined into concentric circular structures. The results showed that the combination of two hydrogels has good properties for swelling, adhesion, in vitro antioxidant, scavenging intracellular ROS, downregulating tumor necrosis factor-α (TNF-α), and enhancing the proliferation and migration of fibroblasts. In a diabetic mouse wound model, the combined application group shortened wound healing time, promoted growth of granulation tissues, and accelerated collagen formation compared with a single hydrogel treatment. The combined use of the two drug-loaded hydrogels has potential application in promoting diabetic wound repair.