Early transcriptomic response of innate immune cells to subcutaneous BCG vaccination of mice
We performed RNA sequencing of innate immune cells derived from murine bone marrow and spleen three days after subcutaneous BCG vaccination. Using fluorescence-activated cell sorting we obtained three cell populations for each mouse from both control and BCG vaccinated groups: bone marrow monocytes and neutrophils and splenic NK-cells. Then double-indexed cDNA libraries for Illumina sequencing from the collected samples were prepared, the resulting cDNA library mix was subjected to NovaSeq 6000 sequencing. This paper describes the collection of 24 RNA sequencing samples comprising 4 sets of immune cell populations obtained from subcutaneously BCG-vaccinated and control mice.
Authors
- A. I. Kuzmich (ORCID: https://orcid.org/0000-0003-3702-3524)
- Olga Rakitina (ORCID: https://orcid.org/0000-0003-4485-0405)
- L. G. Kondratyeva (ORCID: https://orcid.org/0000-0003-3990-8262)
- В. В. Плешкан (ORCID: https://orcid.org/0000-0001-5456-5582)
- Irina Linge (ORCID: https://orcid.org/0000-0003-1535-5800)
- Sofia A. Kondratieva (ORCID: https://orcid.org/0000-0002-1354-5275)
- E. V. Snezhkov
- И. В. Алексеенко (ORCID: https://orcid.org/0000-0002-5357-7685)
- A. V. Sass
Institutions
- Institute of Bioorganic Chemistry (RU)
- Central Scientific Research Institute of Tuberculosis Russian Academy of Medical Sciences (RU)
Publication Details
- Journal
- BMC Research Notes
- Published
- 2024-09-09
- DOI
- https://doi.org/10.1186/s13104-024-06901-w
- Citations
- 2
- Primary Topic
- Immune responses and vaccinations
- Type
- article
- Field-Weighted Citation Impact
- 0.48
Funders
- Russian Science Foundation