Analysis of Synaptic Function Using Single Neuron Culture Preparation

The selection of experimental specimens is critical for elucidating brain function. Specimens used in brain research range from in vivo (individual level) to in vitro (culture level). Recently, in vivo research has been particularly popular, considered an ideal experimental system for observing brain functions in real time and elucidating physiological functions without harming individuals. However, in vivo specimens, which form complex neural circuits, also have drawbacks, including 1) difficulty in data analysis and interpretation of phenomena ; 2) limitations in experimental manipulation and control of neural circuits ; 3) the number of cases cannot be easily obtained due to the required skill in conducting experiments ; and 4) animal welfare issues. On the other hand, an in vitro model introduced in this issue offers the advantage of artificially simplifying the model, enabling a more precise and accurate analysis of brain functions in vitro. This paper presents an autapse culture preparation using single neurons.