Protocol for optimized nasal mucosa sample processing to obtain high-quality scRNA-seq and scATAC-seq data
Examining nasal mucosa samples is crucial for nasal cavity disease research and diagnosis. Simultaneously obtaining high-quality data for single-cell transcriptomics (single-cell RNA sequencing [scRNA-seq]) and epigenomics (single-cell assay for transposase-accessible chromatin using sequencing [scATAC-seq]) of nasal mucosa tissues is challenging. Here, we present a protocol for processing human nasal mucosa samples to obtain data for both scRNA-seq and scATAC-seq. We describe steps for extracting human nasal mucosa tissue, mechanical and enzymatic dissociation, lysis of red blood cells, and a viability assay. We then detail procedures for library preparation and quality control.
Authors
- Xiaojuan Huang (ORCID: https://orcid.org/0000-0001-9938-6553)
- Shuyan Guan (ORCID: https://orcid.org/0000-0002-1119-5646)
- Chuanyu Liu (ORCID: https://orcid.org/0000-0003-2258-0897)
- Yaling Huang (ORCID: https://orcid.org/0000-0003-1408-5490)
- Jinmei Xue (ORCID: https://orcid.org/0000-0002-7428-7812)
- Z.X. Liu
- Ying Liu (ORCID: https://orcid.org/0000-0001-9319-5940)
- Jianhua Yin (ORCID: https://orcid.org/0000-0002-1840-3938)
- Qiaoling Wang (ORCID: https://orcid.org/0000-0002-5135-9895)
- Shikai Han
- Yisha Wu
- Guangpei Cong
Institutions
- BGI Group (China) (CN)
- Shanxi Medical University (CN)
- Second Hospital of Shanxi Medical University (CN)
- BGI Research (CN)
Publication Details
- Journal
- STAR Protocols
- Published
- 2024-09-01
- DOI
- https://doi.org/10.1016/j.xpro.2024.103298
- Citations
- 3
- Primary Topic
- Cancer-related molecular mechanisms research
- Type
- article
- Field-Weighted Citation Impact
- 0.86