Structure Tailoring of Hemicyanine Dyes for <i>In Vivo</i> Shortwave Infrared Imaging
<i>In vivo</i> bioimaging using shortwave infrared (SWIR) (1000-2000 nm) molecular dyes enables deeper penetration and higher contrast compared to visible and near-infrared-I (NIR-I, 700-900 nm) dyes. Developing new SWIR molecules is still quite challenging. This study developed <b>SRHCYs</b>, a panel of fluorescent dyes based on hemicyanine, with adjustable absorbance (830-1144 nm) and emission (886-1217 nm) wavelength. The photophysical attributes of these dyes are precisely tailored by strengthening the donor parts and extending polymethine chains. <b>SRHCY-3</b>, with its clickable azido group, was chosen for high-performance imaging of blood vessels in living mice, enabling the precise detection of brain and lung cancer. The combination of these probes achieved <i>in vivo</i> multicolor imaging with negligible optical crosstalk. This report presents a series of SWIR hemicyanine dyes with promising spectroscopic properties for high-contrast bioimaging and multiplexing detection.
Authors
- Hualong Fu (ORCID: https://orcid.org/0000-0002-7197-8087)
- Jiaming Guo (ORCID: https://orcid.org/0000-0003-3144-589X)
- Mingxi Fang
- Kaixiang Zhou (ORCID: https://orcid.org/0000-0003-4210-6711)
- Yufei Qin (ORCID: https://orcid.org/0000-0001-8873-1825)
- Mengchao Cui (ORCID: https://orcid.org/0000-0002-3488-7864)
- Tianbao Wang (ORCID: https://orcid.org/0000-0001-9327-0146)
- Yuying Li (ORCID: https://orcid.org/0000-0001-9627-6527)
- Yimin Chen (ORCID: https://orcid.org/0000-0002-1602-3253)
- Cheng Liu (ORCID: https://orcid.org/0000-0001-8967-1174)
- Ya‐Jun Liu (ORCID: https://orcid.org/0000-0001-8761-5339)
- Yihan Xu (ORCID: https://orcid.org/0000-0001-6557-1945)
- Longfei Zhang (ORCID: https://orcid.org/0000-0001-7695-9398)
- Yuan Gao (ORCID: https://orcid.org/0000-0003-1910-0071)
- Zihan Xu (ORCID: https://orcid.org/0000-0001-5499-4803)
- Yuqian Qu
- Xiayu Liu
- Yiling Zhu
Institutions
- Xuzhou Medical College (CN)
- Beijing Normal University (CN)
- Stanford University (US)
Publication Details
- Journal
- Journal of Medicinal Chemistry
- Published
- 2024-09-05
- DOI
- https://doi.org/10.1021/acs.jmedchem.4c01662
- Citations
- 11
- Primary Topic
- Nanoplatforms for cancer theranostics
- Type
- article
- Field-Weighted Citation Impact
- 2.03
Funders
- National Natural Science Foundation of China
- Beijing Normal University
- Department of Education of Guangdong Province
- Beijing Municipal Natural Science Foundation