Assessment of Effect of <i>Patol-katurohinyadi Kwatha</i> in Paracetamol Induced Hepato-toxicity in Albino Wistar Rats - An Experimental Study

Background: The present study was planning to assess the hepato-protective and hepato-curative property of Patol-katurohinyadi (PK) kwath, in paracetamol induced hepatotoxicity in albino rats. Hepatotoxicity due to paracetamol and dushi visha concept can be correlated with each other and hepatoprotective formulations mentioned in Ayurveda samhita can play major role in this concept. A classical Ayurvedic formulation Patol-katurohinyadi of Ashtanga hridayam, has been reported to be effective in management of liver related diseases. Objective: To study the hepato-protective and hepato-curative properties of PK Kwatha in paracetamol induced hepatotoxicity in albino rats. Materials and Methods: The experimental study was carried out in albino rats as per CPCSEA guidelines after getting approval from the Institutional Animal Ethics Committee (IAEC). 30 Albino rats, each weighing about 150 gm to 200 gm, were procured from APT Testing and Research Pvt. Ltd. Pune. The rats were housed in the institutional animal house in standard conditions. 30 albino rats were equally divided into five groups having six animals in each group as normal control, disease control, test group, preventive group and curative group. To assess hepato-toxicity, the blood investigations such as RBC, WBC etc. Haematological parameters, biochemical parameters, and histopathological findings were carried out by monitoring rats on 0, 7 and 14 days of study. The results were derived by applying appropriate test to the data. Result: The animals administered with PCM only shows increase in values of SGPT, SPOT, ALP, Albumin, total protein compared to animals of control group. Whereas animals administered with PK Kwatha prevented PCM-induced changes in biochemical parameters and histopathological features. Conclusion: The histopathological results of liver show the potent efficacy of Patol-katurohinyadi kwatha on paracetamol induced hepatotoxicity. Also, we found that Patol-katurohinyadi kwatha as a good hepatoprotective and hepato-curative drug.