Development of a microfluidic SNP assay for lineage discrimination in the endangered hazel dormouse
Abstract The application of Genotyping-by-Sequencing (GBS) approaches is often restricted in wildlife monitoring and conservation genetics, as those fields often rely on noninvasively collected samples with low DNA content. Here we selected a subset of informative single-nucleotide polymorphisms (SNPs) from genome-wide data for lineage discrimination of a locally endangered Eurasian rodent, the hazel dormouse ( Muscardinus avellanarius ), and designed a microfluidic 96 SNP genotyping assay suitable for noninvasively collected samples. Analyses of 43 samples from different European countries confirmed successful discrimination of the Eastern and Western lineage and local substructure within those lineages, proving the suitability of the developed panel for identifying evolutionary significant units and conservation units. Application with 94 hair and scat samples collected in a recent monitoring study on the hazel dormouse in Southern Germany resulted in > 99.5% amplification success showing the applicability of the new tool in genetic wildlife monitoring and conservation studies.
Authors
- Sarah A. Mueller (ORCID: https://orcid.org/0000-0002-6294-0159)
- Sandro Bertolino (ORCID: https://orcid.org/0000-0002-1063-8281)
- Berardino Cocchiararo (ORCID: https://orcid.org/0000-0002-8427-7861)
- Johanna Leyhausen (ORCID: https://orcid.org/0000-0003-1438-4001)
- Joanna Fietz (ORCID: https://orcid.org/0000-0001-6274-7247)
- Goedele Verbeylen (ORCID: https://orcid.org/0000-0002-3967-5721)
- Johan Michaux (ORCID: https://orcid.org/0000-0003-4644-9244)
- Sven Büchner (ORCID: https://orcid.org/0000-0003-1058-1518)
- Alina von Thaden (ORCID: https://orcid.org/0000-0001-9060-0912)
- Carsten Nowak (ORCID: https://orcid.org/0000-0002-3139-1951)
- Johannes Lang (ORCID: https://orcid.org/0000-0002-7387-795X)
- Tobias Beez (ORCID: https://orcid.org/0009-0009-2147-0494)
Institutions
- University of Hohenheim (DE)
- Goethe University Frankfurt (DE)
- University of Liège (BE)
- Justus-Liebig-Universität Gießen (DE)
- Senckenberg Research Institute and Natural History Museum Frankfurt/M (DE)
- LMU Klinikum (DE)
- Accademia Albertina delle Belle Arti (IT)
- Total (Belgium) (BE)
- LOEWE Centre for Translational Biodiversity Genomics (DE)
- Senckenberg Museum für Naturkunde Görlitz (DE)
- Ludwig-Maximilians-Universität München (DE)
Publication Details
- Journal
- Conservation Genetics Resources
- Published
- 2024-09-06
- DOI
- https://doi.org/10.1007/s12686-024-01367-1
- Citations
- 1
- Primary Topic
- Animal Ecology and Behavior Studies
- Type
- article
- Field-Weighted Citation Impact
- 0.46