Metabolic engineering of Saccharomyces cerevisiae for enhanced taxadiene production
Our study highlights the critical role of pathway balance in metabolic engineering, mainly when dealing with toxic molecules like taxadiene. We achieved significant improvements in taxadiene production by employing a combinatorial approach and focusing on balancing the downstream and upstream pathways. These findings emphasize the importance of minor gene expression modification levels to achieve a well-balanced pathway, ultimately leading to enhanced taxadiene accumulation.
Authors
- Hülya Karaca (ORCID: https://orcid.org/0000-0002-6578-1341)
- Yusuf Özkay (ORCID: https://orcid.org/0000-0001-8815-153X)
- Murat Kaya (ORCID: https://orcid.org/0000-0001-6954-2703)
- Serkan Levent (ORCID: https://orcid.org/0000-0003-3692-163X)
- Handan Açelya Kapkaç (ORCID: https://orcid.org/0000-0002-2812-1686)
- Jens Nielsen (ORCID: https://orcid.org/0000-0002-6522-9987)
- Anastasia Krivoruchko (ORCID: https://orcid.org/0000-0003-4536-1814)
- Secil Deniz Ozan
Institutions
- Anadolu University (TR)
- Eskisehir Technical University (TR)
- Chalmers University of Technology (SE)
Publication Details
- Journal
- Microbial Cell Factories
- Published
- 2024-09-06
- DOI
- https://doi.org/10.1186/s12934-024-02512-z
- Citations
- 13
- Primary Topic
- Plant biochemistry and biosynthesis
- Type
- article
- Field-Weighted Citation Impact
- 3.04
Funders
- Anadolu Üniversitesi