Development and Use of Human Recombinant <sup>64</sup>Cu-rHCF as a Kidney Glomerulus-Targeted Contrast Agent for Positron Emission Tomography
In this work, we develop recombinant human cationic ferritin (rHCF) as a contrast agent to detect glomeruli in the kidney using positron emission tomography (PET). We first expressed recombinant human ferritin (rHF) in <i>E. coli</i> and then functionalized and radiolabeled it with Copper-64 (<sup>64</sup>Cu) to form <sup>64</sup>Cu-rHCF. Intravenously injected <sup>64</sup>Cu-rHCF bound to kidney glomeruli and was detected by PET. A subchronic toxicity study after an intravenous injection of rHCF revealed no significant toxicity. The development of rHCF is an important step toward the potential clinical translation of CF to detect the nephron number in humans.
Authors
- Kevin M. Bennett (ORCID: https://orcid.org/0000-0003-1706-4660)
- Edwin J. Baldelomar (ORCID: https://orcid.org/0000-0002-3482-373X)
- Kooresh Isaac Shoghi (ORCID: https://orcid.org/0000-0003-3204-457X)
- David E. Reichert (ORCID: https://orcid.org/0000-0003-3432-8998)
- Daniel L.J. Thorek (ORCID: https://orcid.org/0000-0003-2437-1239)
- Veronica Clavijo Jordan (ORCID: https://orcid.org/0000-0002-1468-164X)
- Hanwen Zhang
- Leslie Dawn Wilson
- Jennifer R. Charlton
- Kasey C. Emoto
- Patrick D. Walker
Institutions
- University of Hawaiʻi at Mānoa (US)
- Harvard University (US)
- Washington University in St. Louis (US)
- Massachusetts General Hospital (US)
- Arkana Laboratories (US)
- OPX Biotechnologies (United States) (US)
- Mallinckrodt (United States) (US)
- Athinoula A. Martinos Center for Biomedical Imaging (US)
- University of Virginia (US)
Publication Details
- Journal
- ACS Applied Bio Materials
- Published
- 2024-09-06
- DOI
- https://doi.org/10.1021/acsabm.4c01027
- Citations
- 1
- Primary Topic
- Radiopharmaceutical Chemistry and Applications
- Type
- article
- Field-Weighted Citation Impact
- 0.40
Funders
- Neurosurgery Research and Education Foundation
- Washington University in St. Louis
- Institute of Clinical and Translational Sciences
- National Institutes of Health
- Center for Cellular Imaging, Washington University
- National Institute of Diabetes and Digestive and Kidney Diseases
- National Center for Advancing Translational Sciences
- Eunice Kennedy Shriver National Institute of Child Health and Human Development
- Office of Research Infrastructure Programs, National Institutes of Health