Preliminary establishment of genetic transformation system for embryogenic callus of Acer truncatum ‘Lihong’

The embryogenic callus was used as the infection material to verify that we successfully transferred the target gene into the embryogenic callus, which means that the genetic transformation system of <i>Acer truncatum</i> embryogenic callus was partially completed, and the infection process could be effectively inhibited. Although there was partial browning, it could continue to proliferate. Therefore, in future experiments, the focus is still to continue to verify the optimal conditions for optimizing the genetic transformation of <i>Acer truncatum</i> embryogenic callus and to solve the problems of difficulty in embryonic callus germination.