Protocol for aerial trapping and analyses of candidate pheromone compounds released by moths via gas chromatography-mass spectrometry
Detection of pheromones is pivotal to chemical ecology and agronomy; however, analytic detection of the volatile pheromone components from odorized air is highly challenging. Here, we introduce a protocol for the detection of airborne pheromones from female moths, which are key models for chemosensory studies. We describe a step-by-step guide from pheromone collection to quantitative estimation of pheromone components. We also detail procedures for gas chromatography-mass spectrometry (GC-MS) analysis. This protocol has potential applications beyond chemosensory research, particularly in environmental chemistry. For complete details on the use and execution of this protocol, please refer to Ghosh et al.<sup>1</sup>.
Authors
- Jérémy Gévar
- Philippe Lucas (ORCID: https://orcid.org/0000-0003-2166-8248)
- Abhishek Chatterjee (ORCID: https://orcid.org/0000-0002-0274-0577)
- S. Ghosh (ORCID: https://orcid.org/0009-0005-3782-1456)
- Antonio Palazzo
Institutions
- Centre National de la Recherche Scientifique (FR)
- Université Paris-Est Créteil (FR)
- Université Paris Cité (FR)
- Sorbonne Université (FR)
- Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement (FR)
- Institut d'écologie et des sciences de l'environnement de Paris (FR)
- Institut de Recherche pour le Développement (FR)
Publication Details
- Journal
- STAR Protocols
- Published
- 2024-09-01
- DOI
- https://doi.org/10.1016/j.xpro.2024.103293
- Citations
- 1
- Primary Topic
- Insect Pheromone Research and Control
- Type
- article
- Field-Weighted Citation Impact
- 0.54
Funders
- Institut National de Recherche en Sciences et Technologies pour l'Environnement et l'Agriculture
- Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement