Evaluation of amlodipine against strains of <i>Candida</i> spp. in planktonic cells, developing biofilms and mature biofilms
<b>Aim:</b> To evaluate the antifungal activity of amlodipine against strains of <i>Candida</i> spp. and to its possible mechanism of action.<b>Methods:</b> Broth microdilution tests were used to determine the minimum inhibitory concentration, while the synergistic activity was evaluated by calculating the fractional inhibitory concentration index. The action of amlodipine against biofilms was determined using the MTT assay and its possible mechanism of action was investigated through flow cytometry tests.<b>Results:</b> Amlodipine showed MICs ranging from 62.5 to 250 μg/ml, in addition to action against pre-formed and forming biofilms, with reductions between 50 and 90%. Amlodipine increases the externalization of phosphatidylserine and reduces the cell viability of fungal cells, suggesting apoptosis.<b>Conclusion:</b> Amlodipine had good antifungal activity against planktonic cells and biofilms of <i>Candida</i> spp., by leading the cells to apoptosis.
Authors
- Islay Lima Magalhães
- Fátima Daiana Dias Barroso (ORCID: https://orcid.org/0000-0002-3275-9754)
- Cecília Rocha da Silva (ORCID: https://orcid.org/0000-0003-4964-351X)
- Helaine Almeida Queiroz (ORCID: https://orcid.org/0000-0003-2468-143X)
- Lara Elloyse Almeida Moreira (ORCID: https://orcid.org/0000-0003-0285-1258)
- Daniel Sampaio Rodrigues (ORCID: https://orcid.org/0000-0001-8496-8243)
- Vitória Pessoa de Farias Cabral (ORCID: https://orcid.org/0000-0002-6408-7200)
- Bruno Coêlho Cavalcanti (ORCID: https://orcid.org/0000-0002-0725-8392)
- Lívia Gurgel do Amaral Valente Sá (ORCID: https://orcid.org/0000-0002-0619-2782)
- Lisandra Juvêncio da Silva
- Amanda Dias Barbosa (ORCID: https://orcid.org/0000-0002-4104-5518)
- Hélio Vitoriano Nobre Júnior (ORCID: https://orcid.org/0000-0003-0156-5882)
- João Batista de Andrade Neto (ORCID: https://orcid.org/0000-0002-3180-3012)
- Manoel Odorico de Moraes (ORCID: https://orcid.org/0000-0003-3378-8722)
- Leilson Carvalho de Oliveira (ORCID: https://orcid.org/0009-0005-4545-9442)
- Érica Rayanne Motta da Costa
Institutions
- Universidade Federal do Ceará (BR)
- Unichristus (BR)
- International Drug Development (FR)
- Ashland (United States) (US)
Publication Details
- Journal
- Future Microbiology
- Published
- 2024-09-05
- DOI
- https://doi.org/10.1080/17460913.2024.2390286
- Citations
- 1
- Primary Topic
- Antifungal resistance and susceptibility
- Type
- article
- Field-Weighted Citation Impact
- 0.39