CORRELATION BETWEEN PRE AND POST TREATMENT EFFECT OF METHOTREXATE AND ANTI-OXIDANT STATUS IN RHEUMATOID ARTHRITIS PATIENTS

Rheumatoid Arthritis (RA) is a chronic inflammatory disorder characterized by oxidative stress and joint damage. This study evaluates the biochemical impact of Methotrexate (MTX) treatment on RA patients, focusing on oxidative stress markers, antioxidant activity, and overall disease management. Analysis of 60 RA patients before and after MTX treatment revealed significant biochemical changes. Post-treatment, Malondialdehyde (MDA), a marker of oxidative stress, was elevated, while Glutathione (GSH) levels increased, indicating some restoration of antioxidant defense. However, catalase and superoxide dismutase (SOD) activities were significantly reduced, reflecting persistent oxidative imbalance. Non-enzymatic antioxidants, including vitamins A, C, and E, showed substantial improvement post-treatment. Elevated Advanced Oxidation Protein Products (AOPP) and reduced nitric oxide (NO) levels post-treatment suggest decreased inflammatory activity. Electrolyte levels, specifically sodium and potassium, were notably altered, indicating potential disruptions in homeostasis. Additionally, increased urea and creatinine levels post-treatment point to potential renal dysfunction. This study underscores Methotrexate's role in modulating oxidative stress and inflammation in RA, highlighting the importance of monitoring biochemical parameters for effective disease management and the need for further research into long-term treatment effects and management strategies.