Synergistic effect of new pyrimidine derivative with oxacilin against methicillin-resistant <i>Staphylococcus aureus</i>

<b>Aim:</b> This work aimed to synthesize a new pyrimidine <b>PYB01</b> with potential application against antimicrobial resistance.<b>Materials & methods: PYB01</b> was synthesized through condensation reaction between <b>3a</b> and <b>3b</b>. The antimicrobial evaluation was carried out using the microdilution method in Mueller-Hinton Agar and <i>in silico</i> predictions using different software.<b>Results: PYB01</b> has moderate antibiotic activity and high capacity to efficiently modulate antibiotic resistance in <i>Staphylococcus aureus</i>. <i>In silico</i> evaluations demonstrated that <b>PYB01</b> is probably an allosteric inhibitor of Protein Binding Penicilin 2a and modulates the action of oxacillin by decreasing the minimum inhibitory concentration by 64-times. <b>PYB01</b> demonstrate a good pharmacokinetic profile and toxicological.<b>Conclusion: PYB01</b> has great potential to go further in investigating its use against antimicrobial resistance.