A two-step strategy to expand primary human hepatocytes in vitro with efficient metabolic and regenerative capacities
This study presents a two-step strategy that initially induces PHHs into a proliferative state (ProHPLCs) to ensure sufficient cell quantity, followed by the maturation of ProHPLCs into fully functional hepatocytes to guarantee optimal cell quality. This approach offers a promising means of producing large numbers of seeding cells for hepatocyte-based applications.
Authors
- Jihang Shi
- Simeng Yi
- Yong Jiang (ORCID: https://orcid.org/0000-0001-5540-377X)
- Jianhua Peng (ORCID: https://orcid.org/0000-0003-3348-9538)
- Shigang Yin (ORCID: https://orcid.org/0000-0003-0955-917X)
- Shichun Lu (ORCID: https://orcid.org/0000-0003-3278-0534)
- Guangya Li
- Huangfan Xie (ORCID: https://orcid.org/0000-0002-6342-4316)
- Hongkui Deng (ORCID: https://orcid.org/0000-0003-3045-4428)
- Bingqing Xie (ORCID: https://orcid.org/0000-0001-9433-1641)
- Nan Jiang (ORCID: https://orcid.org/0000-0003-4913-4395)
- Xi Kong (ORCID: https://orcid.org/0009-0008-6993-3251)
- Yunxi Fu
Institutions
- Peking University (CN)
- Chinese PLA General Hospital (CN)
- Southwest Medical University (CN)
- Affiliated Hospital of Southwest Medical University (CN)
Publication Details
- Journal
- Stem Cell Research & Therapy
- Published
- 2024-09-04
- DOI
- https://doi.org/10.1186/s13287-024-03911-0
- Citations
- 2
- Primary Topic
- Liver physiology and pathology
- Type
- article
- Field-Weighted Citation Impact
- 0.74
Funders
- National Natural Science Foundation of China
- Peking University
- Southwest Medical University
- Luzhou Science and Technology Bureau