Assessment of transthyretin instability in patients with wild-type transthyretin amyloid cardiomyopathy
The pathophysiology of variant transthyretin (TTR) amyloidosis (ATTRv) is associated with destabilizing mutations in the TTR tetramer. However, why TTR with a wild-type genetic sequence misfolds and aggregates in wild-type transthyretin amyloidosis (ATTRwt) is unknown. Here, we evaluate kinetic TTR stability with a newly developed ELISA system in combination with urea-induced protein denaturation. Compared with that in control patients, endogenous TTR in patients with wild-type transthyretin amyloid cardiomyopathy (ATTRwt-CM) exhibited thermodynamic instability, indicating that circulating TTR instability may be associated with the pathogenesis of ATTRwt as well as ATTRv. Our findings provide new insight into the underlying mechanisms of ATTRwt.
Authors
- Makoto Nishimori (ORCID: https://orcid.org/0000-0002-1366-9948)
- Junko Asakura
- Amane Harada (ORCID: https://orcid.org/0000-0002-6455-9765)
- Takuya Iino
- Masakazu Shinohara (ORCID: https://orcid.org/0000-0001-8724-0071)
- Hidekazu Tanaka (ORCID: https://orcid.org/0000-0002-1642-7039)
- Sachiko Yoshikawa
- Manabu Nagao (ORCID: https://orcid.org/0000-0001-7269-4513)
- Ryuji Toh (ORCID: https://orcid.org/0000-0002-7204-6695)
- Ken‐ichi Hirata (ORCID: https://orcid.org/0000-0002-6782-3031)
- Tatsuro Ishida (ORCID: https://orcid.org/0000-0002-1724-0057)
- Shunsuke Watanabe (ORCID: https://orcid.org/0000-0001-7803-396X)
Institutions
- Sysmex (Japan) (JP)
- Kobe University (JP)
Publication Details
- Journal
- Scientific Reports
- Published
- 2024-09-03
- DOI
- https://doi.org/10.1038/s41598-024-71446-8
- Citations
- 4
- Primary Topic
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Type
- article
- Field-Weighted Citation Impact
- 0.94
Funders
- Ministry of Education, Culture, Sports, Science and Technology
- Japan Heart Foundation