Fibre-specific mitochondrial protein abundance is linked to resting and post-training mitochondrial content in the muscle of men
Analyses of mitochondrial adaptations in human skeletal muscle have mostly used whole-muscle samples, where results may be confounded by the presence of a mixture of type I and II muscle fibres. Using our adapted mass spectrometry-based proteomics workflow, we provide insights into fibre-specific mitochondrial differences in the human skeletal muscle of men before and after training. Our findings challenge previous conclusions regarding the extent of fibre-type-specific remodelling of the mitochondrial proteome and suggest that most baseline differences in mitochondrial protein abundances between fibre types reported by us, and others, might be due to differences in total mitochondrial content or a consequence of adaptations to habitual physical activity (or inactivity). Most training-induced changes in different mitochondrial functional groups, in both fibre types, were no longer significant in our study when normalised to changes in markers of mitochondrial content.
Authors
- Georg Ramm (ORCID: https://orcid.org/0000-0003-3596-2288)
- Javier Botella (ORCID: https://orcid.org/0000-0001-9722-8519)
- David A. Stroud (ORCID: https://orcid.org/0000-0002-2048-3383)
- Viola Oorschot (ORCID: https://orcid.org/0000-0002-8122-8271)
- Cheng Huang (ORCID: https://orcid.org/0000-0002-4575-1233)
- Elizabeth G. Reisman (ORCID: https://orcid.org/0000-0002-5791-3324)
- David John Bishop (ORCID: https://orcid.org/0000-0002-6956-9188)
- Cesare Granata (ORCID: https://orcid.org/0000-0002-3509-6001)
- Nikeisha J. Caruana (ORCID: https://orcid.org/0000-0002-0817-1686)
- Ralf B. Schittenhelm (ORCID: https://orcid.org/0000-0001-8738-1878)
- Owala S. Chandrasiri
Institutions
- Royal Children's Hospital (AU)
- Deakin University (AU)
- The University of Melbourne (AU)
- Australian Regenerative Medicine Institute (AU)
- Victorian Clinical Genetics Services (AU)
- European Molecular Biology Laboratory (DE)
- Murdoch Children's Research Institute (AU)
- Deutsches Diabetes-Zentrum e.V. (DE)
- German Center for Diabetes Research (DE)
- Heinrich Heine University Düsseldorf (DE)
- Monash University (AU)
- Victoria University (AU)
- Australian Catholic University (AU)
Publication Details
- Journal
- Nature Communications
- Published
- 2024-09-03
- DOI
- https://doi.org/10.1038/s41467-024-50632-2
- Citations
- 20
- Primary Topic
- Muscle metabolism and nutrition
- Type
- article
- Field-Weighted Citation Impact
- 6.19