A marker-free genetic manipulation method for Glaesserella parasuis strains developed by alternately culturing transformants at 37°C and 30°C
In conclusion, this newly developed gene knockout system for G. parasuis presents a valuable tool for advancing research on this organism.
Authors
- Dongfang Wu (ORCID: https://orcid.org/0000-0002-9811-2291)
- Yuping Song
- Xiaojuan Xu (ORCID: https://orcid.org/0000-0002-2088-9201)
- Xuwang Cai (ORCID: https://orcid.org/0000-0003-1026-9663)
- Jing Xiao (ORCID: https://orcid.org/0000-0002-7006-6211)
- Hongbo Zhou (ORCID: https://orcid.org/0000-0002-3954-3972)
- Huanchun Chen (ORCID: https://orcid.org/0000-0003-0680-5953)
- Yuxin Wang (ORCID: https://orcid.org/0000-0002-4490-3995)
Institutions
- Huazhong Agricultural University (CN)
- Yebio Bioengineering (China) (CN)
- Center of Hubei Cooperative Innovation for Emissions Trading System (CN)
Publication Details
- Journal
- BMC Biotechnology
- Published
- 2024-09-03
- DOI
- https://doi.org/10.1186/s12896-024-00887-w
- Citations
- 1
- Primary Topic
- Viral Infectious Diseases and Gene Expression in Insects
- Type
- article
- Field-Weighted Citation Impact
- 0.23
Funders
- National Key Research and Development Program of China