Anti-Cancer Activity Of The Combination Of Marine Mineral Consentrates And α Mangostin On Prostate Cancer Line DU 145 And HEK 293 By WST-8 Method

Marine mineral concentrates have good activity against normal cells, but are less effective against cancer cells. So in this research, a combination of sea mineral concentrations was carried out with natural ingredients that have anti-cancer potential, one of which was α-mangosteen. This study aims to determine the IC50 of the combination of α-mangosteen, marine mineral concentrate, the combination of α-mangostin & marine mineral concentrate when compared with cisplatin and evaluate the activity of α-mangostin, marine mineral concentrate and the combination of α-mangostin and marine mineral concentrate when compared with ciplatin against the DU 145 and HEK 293 cancer cell lines. The method used was cytotoxicity testing using the WST-8 method. The results of each test substance were α-Mangostin 16.89 ppm, the combination of α-Mangostin-KML with a concentration of 12.5 ppm, namely 1,732 ppm, the combination of α-Mangostin-KML with a concentration of 25 ppm, namely 4,930 ppm, the combination of α-Mangostin- KML with a concentration of 50 ppm 5,194 ppm, and cisplatin 4,649 ppm. The results of data analysis show significant differences for all samples. Conclusion α-Mangostin helps sea mineral concentrate to increase the cytotoxic effect on DU 145 cancer cells and sea mineral concentrate helps reduce the cytotoxic effect of α-Mangostin on normal HEK-293 cells.