Reclassification of Butyrivibrio crossotus Moore et al. 1976 (Approved Lists 1980) into a novel genus as Eshraghiella crossota gen. nov., comb. nov.

The reclassification of <i>Butyrivibrio crossotus</i> Moore <i>et al</i>. 1976 (Approved Lists 1980) as <i>Eshraghiella crossota</i> gen. nov., comb. nov. is proposed within the family <i>Lachnospiraceae</i>. This reclassification is based on differences revealed through the analysis of 16S rRNA, <i>groEL</i>, <i>recA</i>, and <i>rpoB</i> genes, as well as genome sequences, distinguishing it from other <i>Butyrivibrio</i> species. Comparative analysis showed that <i>B. crossotus</i> exhibited digital DNA-DNA hybridization (dDDH) values of 19.40-27.20% and average nucleotide identities based on blast (ANIb) values of 67.06-67.64% with other <i>Butyrivibrio</i> species. These values are significantly below the species delineation thresholds (dDDH, 70%; ANIb, 95-96%), justifying the proposed reclassification. Additionally, the results of the average amino acid identity (AAI) analysis indicated that this species shares 59.22-60.17% AAI with the other species of the genus <i>Butyrivibrio</i>, which is below the AAI threshold (65%) for a genus boundary. In addition, biochemical and morphological characteristics also support the proposal that this species is different from other species of the genus <i>Butyrivibrio</i>. The type strain is ATCC 29175^T (DSM 2876^T=T9-40A^T).