Metabolic engineering of Escherichia coli for high-level production of benzyl acetate from glucose
We were able to establish the whole pathway for benzyl acetate based on the CoA-dependent β-oxidation in single strain for the first time. The highest titer for benzyl acetate produced from glucose by E. coli is reported. Moreover, cinnamyl acetate production as an unwanted by-product was very low. Results provided novel information regarding the engineering benzyl acetate production in microorganisms.
Authors
- Yibin Zhuang (ORCID: https://orcid.org/0000-0001-6369-022X)
- Zhanzhao Cui
- Hua Yin (ORCID: https://orcid.org/0000-0002-2793-0860)
- Cuiying Zhang (ORCID: https://orcid.org/0000-0003-4926-4202)
- Qin Ke
- Yaju Xue
- Chang Liu (ORCID: https://orcid.org/0000-0003-0650-1773)
- Tao Liu (ORCID: https://orcid.org/0000-0001-9529-6550)
Institutions
- Tianjin University of Science and Technology (CN)
- Tianjin University of Technology (CN)
- Chinese Academy of Sciences (CN)
- Intelligent Synthetic Biology Center (KR)
- Tianjin Institute of Industrial Biotechnology (CN)
Publication Details
- Journal
- Microbial Cell Factories
- Published
- 2024-09-03
- DOI
- https://doi.org/10.1186/s12934-024-02513-y
- Citations
- 5
- Primary Topic
- Microbial Metabolic Engineering and Bioproduction
- Type
- article
- Field-Weighted Citation Impact
- 1.17
Funders
- National Natural Science Foundation of China
- Chinese Academy of Sciences
- National Key Research and Development Program of China