Metalloproteomics Reveals Multi-Level Stress Response in Escherichia coli When Exposed to Arsenite

The <i>arsRBC</i> operon encodes a three-protein arsenic resistance system. ArsR regulates the transcription of the operon, while ArsB and ArsC are involved in exporting trivalent arsenic and reducing pentavalent arsenic, respectively. Previous research into <i>Agrobacterium tumefaciens</i> 5A has demonstrated that ArsR has regulatory control over a wide range of metal-related proteins and metabolic pathways. We hypothesized that ArsR has broad regulatory control in other Gram-negative bacteria and set out to test this. Here, we use differential proteomics to investigate changes caused by the presence of the <i>arsR</i> gene in human microbiome-relevant <i>Escherichia coli</i> during arsenite (As^III) exposure. We show that ArsR has broad-ranging impacts such as the expression of TCA cycle enzymes during As^III stress. Additionally, we found that the Isc [Fe-S] cluster and molybdenum cofactor assembly proteins are upregulated regardless of the presence of ArsR under these same conditions. An important finding from this differential proteomics analysis was the identification of response mechanisms that were strain-, ArsR-, and arsenic-specific, providing new clarity to this complex regulon. Given the widespread occurrence of the <i>arsRBC</i> operon, these findings should have broad applicability across microbial genera, including sensitive environments such as the human gastrointestinal tract.