Paracoccidioides lutzii Infects Galleria mellonella Employing Formamidase as a Virulence Factor

The formamidase (FMD) enzyme plays an important role in fungal thriving by releasing a secondary nitrogen source as a product of its activity. In Paracoccidioides species, previous studies have demonstrated the upregulation of this enzyme in a wide range of starvation and infective-like conditions. However, Paracoccidioides lutzii formamidase has not yet been defined as a virulence factor. Here, by employing in vivo infections using an fmd -silenced strain in Galleria mellonella larvae model, we demonstrate the influence of formamidase in P . lutzii ’s immune stimulation and pathogenicity. The formamidase silencing resulted in improper arrangement of the nodules, poor melanogenesis and decreased fungal burden. Thus, we suggest that formamidase may be a piece composing the process of molecular recognition by Galleria immune cells. Furthermore, formamidase silencing doubled the observed survival rate of the larvae, demonstrating its importance in fungal virulence in vivo . Therefore, our findings indicate that formamidase contributes to Galleria ’s immune incitement and establishes the role of this enzyme as a P . lutzii virulence factor.