Potent Cytotoxicity and Nitric Oxide Suppression of Compounds Derived from Kaempferia elegans Rhizomes: Molecular Modeling on EGFR Inhibition

A new naturally occurring diarylheptanoid, (1E,4Z,6E)-5-hydroxy-1,7-diphenylhepta-1,4,6-trien-3one (3), was isolated from the rhizomes of K. elegans along with six known compounds, flavokawain B (1), 5,6dehydrokawain (2), pinocembrin (4), cardamonin (5), alpinetin (6), and crotepoxide (7), among which compound 6 had not previously been isolated from this plant species.Two chalcones, flavokawain B (1) and cardamonin (5) were active against nitric oxide (NO) radicals released from LPS-induced RAW264.7 macrophages, resulting in 91.58% and 98.68% inhibition of NO production, respectively.Furthermore, compounds 1 and 5 showed superior cytotoxicity against MCF-7 (IC50 = 23.07 and 20.84 M) and MDA-MB-231 (IC50 = 21.77and 26.64 M) cell lines, respectively.In silico molecular modeling studies of the most active compounds 1 and 5 against epidermal growth factor receptors (EGFR) suggested that - interactions with residues on the EGFR protein contributed to their anticancer properties.The results suggest that cardamonin (5) could be a promising candidate for further development of anti-inflammatory and anticancer agents.