Genetic evolutionary analysis of a strain of Senecavirus A in Anhui and the establishment of its detection method
The complete genome of SVA-CH-AHAU-1 is 7286 bp, including a 5' non-coding region (UTR), an open reading frame (ORF) of 6546 nucleotides, encoding 2182 amino acids (aa), and a 3' UTR with Poly(A) features, and phylogenetic analysis showed that this isolate had the highest nucleotide homology (97.9 %) with the US isolate US-15-41901SD. In this study, the virulent strain SVA-CH-AHAU-1 was found to recombine in the ORF region with isolates SVA-CH-SDGT-2017 and SVA/Canada/ON/FMA-2015-0024 T2/2015. The complete genome has been submitted to GeneBank with the accession number OM654411. In addition, our results suggest that the established nano-PCR assay can be used as an economical, reliable and sensitive method for the field diagnosis of SVA method, especially in resource-limited areas.
Authors
- Haixiao Shen
- Yuting Xue (ORCID: https://orcid.org/0000-0002-9463-2951)
- Huihui Sun (ORCID: https://orcid.org/0000-0002-8298-8766)
- Xuan Wang (ORCID: https://orcid.org/0000-0002-9679-9771)
- Lijun Chen (ORCID: https://orcid.org/0000-0001-9374-6466)
- Jie Li (ORCID: https://orcid.org/0000-0002-7395-8207)
- Liang Li (ORCID: https://orcid.org/0000-0003-1002-4228)
- Haicheng Lin
- P. Sun
- Zongyi Bo
Institutions
- Nanjing Agricultural University (CN)
- Anhui Agricultural University (CN)
- Yangzhou University (CN)
Publication Details
- Journal
- Infection Genetics and Evolution
- Published
- 2024-09-03
- DOI
- https://doi.org/10.1016/j.meegid.2024.105665
- Citations
- 1
- Primary Topic
- Plant and Fungal Interactions Research
- Type
- article
- Field-Weighted Citation Impact
- 0.62
Funders
- National Natural Science Foundation of China
- Anhui Agricultural University
- Anhui Provincial Department of Education