Variation in the prion protein gene (PRNP) open reading frame sequence in French cervids

The recent emergence of chronic wasting disease (CWD) in Europe has become a new public health risk for monitoring of wild and farmed cervids. This disease, due to prions, has proliferated in North America in a contagious manner. In several mammalian species, polymorphisms in the prion protein gene (PRNP) play a crucial role in the susceptibility to prions and their spread. To obtain a reliable picture of the distribution of PRNP polymorphisms in the two most common cervid species in France, we sequenced the open reading frame (ORF) of this gene in 2114 animals, 1116 roe deer (Capreolus capreolus) and 998 red deer (Cervus elaphus). Selection criteria such as historical origin, spatial distribution and sex ratio have been integrated to establish this sample collection. Except for one heterozygous animal with a non-synonymous mutation at codon 37 (G37A), all the 1116 French roe deer were monomorphic. Red deer showed greater variation with two non-synonymous substitutions (T98A; Q226E), three synonymous substitutions (codons 21, 78 and 136) and a new 24pb deletion (Δ<sub>69-77</sub>). We found significant regional variations between French regions in the frequency of the identified substitutions. After cloning of the PRNP ORF from animals presenting multiple non-synonymous polymorphisms, we identified six haplotypes and obtained a total of twelve genotypes. As in other European countries, we highlighted the apparent homogeneity of PRNP in the French roe deer and the existence of a greater diversity in the red deer. These results were in line with European phylogeographic studies on these two species.

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Journal
Veterinary Research
Published
2024-09-03
DOI
https://doi.org/10.1186/s13567-024-01362-2
Primary Topic
Prion Diseases and Protein Misfolding
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article
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article

Variation in the prion protein gene (PRNP) open reading frame sequence in French cervids

Nathalie Daniel, Vincent Béringue, Dénis Laloë, Christine Saint‐Andrieux et al.
1 links
Veterinary Research
Prion Diseases and Protein Misfolding
article

Variation in the prion protein gene (PRNP) open reading frame sequence in French cervids

Nathalie Daniel, Vincent Béringue, Dénis Laloë, Christine Saint‐Andrieux, Maryline Pellerin, Johan Castille, Olivier Andréoletti, Aurélie Barboiron, Bruno Passet, Johann Laubier, Jean-Luc Vilotte, Sandrine Floriot, Vincent Bourret, Katayoun Moazami‐Goudarzi, Sonja Ernst, Anne Van De Wiele, Emma Meloni, Joël Merlet, Hélène Verheyden, Fiona Houston
article en

Abstract

The recent emergence of chronic wasting disease (CWD) in Europe has become a new public health risk for monitoring of wild and farmed cervids. This disease, due to prions, has proliferated in North America in a contagious manner. In several mammalian species, polymorphisms in the prion protein gene (PRNP) play a crucial role in the susceptibility to prions and their spread. To obtain a reliable picture of the distribution of PRNP polymorphisms in the two most common cervid species in France, we sequenced the open reading frame (ORF) of this gene in 2114 animals, 1116 roe deer (Capreolus capreolus) and 998 red deer (Cervus elaphus). Selection criteria such as historical origin, spatial distribution and sex ratio have been integrated to establish this sample collection. Except for one heterozygous animal with a non-synonymous mutation at codon 37 (G37A), all the 1116 French roe deer were monomorphic. Red deer showed greater variation with two non-synonymous substitutions (T98A; Q226E), three synonymous substitutions (codons 21, 78 and 136) and a new 24pb deletion (Δ<sub>69-77</sub>). We found significant regional variations between French regions in the frequency of the identified substitutions. After cloning of the PRNP ORF from animals presenting multiple non-synonymous polymorphisms, we identified six haplotypes and obtained a total of twelve genotypes. As in other European countries, we highlighted the apparent homogeneity of PRNP in the French roe deer and the existence of a greater diversity in the red deer. These results were in line with European phylogeographic studies on these two species.

Veterinary ResearchVol. 55(1)
Friedrich-Loeffler-Institut (DE), Roslin Institute (GB), École Nationale Vétérinaire de Toulouse (FR), Université de Versailles Saint-Quentin-en-Yvelines (FR), Institut National Polytechnique de Toulouse (FR), AgroParisTech (FR), Université Paris-Saclay (FR), Microbiologie de l’alimentation au service de la santé (FR), Edinburgh College (GB), Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement (FR), French National Agency for Water and Aquatic Environments (FR), Virologie et Immunologie Moléculaires (FR), Office National de la Chasse et de la Faune Sauvage (FR), Génétique Animale et Biologie Intégrative (FR), Interactions hôtes-agents pathogènes (FR), University of Edinburgh (GB)
Animal Health and Welfare ERA-Net, European Commission, Agence Nationale de la Recherche, Biotechnology and Biological Sciences Research Council
Openalex Percentile: Top 87%
Prion Diseases and Protein Misfolding
DOIOpen Access PDFOpenAlex
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