Bullet-DNP Enables NMR Spectroscopy of Pyruvate and Amino Acids at Nanomolar to Low Micromolar Concentrations
Hyperpolarized pyruvate is a widely used marker to track metabolism in vivo and a benchmark molecule for hyperpolarization methods. Here, we show how a combination of improved bullet-DNP instrumentation, an optimized sample preparation and a further sensitivity increase via a <sup>13</sup>C-<sup>1</sup>H polarization transfer after dissolution enable the observation of pyruvate at a concentration of 250 nM immediately after dissolution. At this concentration, the experiment employs a total mass of pyruvate of only 20 ng or 180 pmol. If the concentration is increased to 45 μM, pyruvate may be detected 1 min after dissolution with a signal-to-noise ratio exceeding 50. The procedure can be extended to observe a mixture of amino acids at low micromolar concentrations.
Authors
- Karel Kouřil (ORCID: https://orcid.org/0000-0003-4884-1637)
- Pooja Narwal (ORCID: https://orcid.org/0000-0003-3425-8942)
- Sami Jannin (ORCID: https://orcid.org/0000-0002-8877-4929)
- Alvar D. Gossert (ORCID: https://orcid.org/0000-0001-7732-495X)
- Masoud Minaei (ORCID: https://orcid.org/0000-0001-6233-416X)
- Benno Meier (ORCID: https://orcid.org/0000-0003-2258-1507)
- Nils Lorz (ORCID: https://orcid.org/0009-0005-5438-9028)
Institutions
- Université Claude Bernard Lyon 1 (FR)
- Karlsruhe Institute of Technology (DE)
- École Normale Supérieure de Lyon (FR)
- Centre National de la Recherche Scientifique (FR)
- ETH Zurich (CH)
- Centre de RMN à Très Hauts Champs de Lyon (FR)
Publication Details
- Journal
- Analytical Chemistry
- Published
- 2024-09-03
- DOI
- https://doi.org/10.1021/acs.analchem.4c00618
- Citations
- 6
- Primary Topic
- Advanced NMR Techniques and Applications
- Type
- article
- Field-Weighted Citation Impact
- 1.44
Funders
- Helmholtz-Gemeinschaft
- Deutsche Forschungsgemeinschaft
- H2020 European Research Council