<i>Bacillus subtilis</i> grown in a “breathing” vessel without sparger aeration
Here we present a "breathing" vessel consisting of expanded polytetrafluoroethylene, which allows gas exchange but no liquid permeation. The bacterial culture inside needs only agitation to promote air supply. Using this setup, a Bacillus subtilis cell factory for scyllo-inositol production grew to produce scyllo-inositol efficiently. The results indicate that our approach represents a sustainable "greener" approach for the cell factory.
Authors
- Ryosuke Sato (ORCID: https://orcid.org/0000-0001-5899-3410)
- Shu Ishikawa (ORCID: https://orcid.org/0000-0002-2239-1116)
- Ayşegül Öktem (ORCID: https://orcid.org/0000-0001-7828-999X)
- Jan Maarten van Dijl (ORCID: https://orcid.org/0000-0002-5688-8438)
- Ken‐ichi Yoshida (ORCID: https://orcid.org/0000-0002-3383-4664)
- Masato Yotsuya
- Kyosuke Yokoyama
Institutions
- University Medical Center Groningen (NL)
- University of Groningen (NL)
- Kobe University (JP)
Publication Details
- Journal
- Bioscience Biotechnology and Biochemistry
- Published
- 2024-09-02
- DOI
- https://doi.org/10.1093/bbb/zbae120
- Citations
- 1
- Primary Topic
- Probiotics and Fermented Foods
- Type
- article
- Field-Weighted Citation Impact
- 0.35
Funders
- H2020 Marie Skłodowska-Curie Actions