Emerin mislocalization during chromatin bridge resolution can drive prostate cancer cell invasiveness in a collagen-rich microenvironment

Micronuclei (MN) can form through many mechanisms, including the breakage of aberrant cytokinetic chromatin bridges. The frequent observation of MN in tumors suggests that they might not merely be passive elements but could instead play active roles in tumor progression. Here, we propose a mechanism through which the presence of micronuclei could induce specific phenotypic and functional changes in cells and increase the invasive potential of cancer cells. Through the integration of diverse in vitro imaging and molecular techniques supported by clinical samples from patients with prostate cancer (PCa) defined as high-risk by the D'Amico classification, we demonstrate that the resolution of chromosome bridges can result in the accumulation of Emerin and the formation of Emerin-rich MN. These structures are negative for Lamin A/C and positive for the Lamin-B receptor and Sec61β. MN can act as a protein sinks and result in the pauperization of Emerin from the nuclear envelope. The Emerin mislocalization phenotype is associated with a molecular signature that is correlated with a poor prognosis in PCa patients and is enriched in metastatic samples. Emerin mislocalization corresponds with increases in the migratory and invasive potential of tumor cells, especially in a collagen-rich microenvironment. Our study demonstrates that the mislocalization of Emerin to MN results in increased cell invasiveness, thereby worsening patient prognosis.

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Publication Details

Journal
Experimental & Molecular Medicine
Published
2024-09-01
DOI
https://doi.org/10.1038/s12276-024-01308-w
Citations
4
Primary Topic
Nuclear Structure and Function
Type
article
Field-Weighted Citation Impact
0.94

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Emerin mislocalization during chromatin bridge resolution can drive prostate cancer cell invasiveness in a collagen-rich microenvironment

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4 citations
Experimental & Molecular Medicine
Nuclear Structure and Function
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article

Emerin mislocalization during chromatin bridge resolution can drive prostate cancer cell invasiveness in a collagen-rich microenvironment

Marcin Matuszewski, Jolanta Szade, Zeno Lavagnino, Tomasz Wenta, Kevin Miszewski, Sara Barozzi, Rebecca Bertolio, Michał Bieńkowski, Natalia Bednarz‐Knoll, Galina V. Beznoussenko, Michał Rychłowski, Luca Braga, Alexander A. Mirоnоv, Kamil Kowalski, Marta Popęda, Giannino Del Sal, Anna Joanna Zaczek, Kamil Myszczyński, Paolo Maiuri, Paulina Nastały, J R Richert
article en
4 citations

Abstract

Micronuclei (MN) can form through many mechanisms, including the breakage of aberrant cytokinetic chromatin bridges. The frequent observation of MN in tumors suggests that they might not merely be passive elements but could instead play active roles in tumor progression. Here, we propose a mechanism through which the presence of micronuclei could induce specific phenotypic and functional changes in cells and increase the invasive potential of cancer cells. Through the integration of diverse in vitro imaging and molecular techniques supported by clinical samples from patients with prostate cancer (PCa) defined as high-risk by the D'Amico classification, we demonstrate that the resolution of chromosome bridges can result in the accumulation of Emerin and the formation of Emerin-rich MN. These structures are negative for Lamin A/C and positive for the Lamin-B receptor and Sec61β. MN can act as a protein sinks and result in the pauperization of Emerin from the nuclear envelope. The Emerin mislocalization phenotype is associated with a molecular signature that is correlated with a poor prognosis in PCa patients and is enriched in metastatic samples. Emerin mislocalization corresponds with increases in the migratory and invasive potential of tumor cells, especially in a collagen-rich microenvironment. Our study demonstrates that the mislocalization of Emerin to MN results in increased cell invasiveness, thereby worsening patient prognosis.

Experimental & Molecular MedicineVol. 56(9)
University of Trieste (IT), University of Gdańsk (PL), IFOM (IT), University of Naples Federico II (IT), Gdańsk Medical University (PL), International Centre for Genetic Engineering and Biotechnology (IT)
Narodowe Centrum Nauki, Associazione Italiana per la Ricerca sul Cancro
No poverty
Openalex Percentile: Top 25%
Nuclear Structure and Function
0.94
DOIOpen Access PDFOpenAlex
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