Antibiotics stimulates the development of persistent cells in biofilms of <i>Candida albicans</i> bloodstream isolates
<i>Candida albicans</i> invasive candidiasis is considered a global health problem. In such cases, biofilm formation on implanted devices represents a therapeutic challenge and the presence of metabolically inactive persistent cells (PCs) in these communities increases their tolerance to fungicidal drugs. This study investigated the influence of amoxicillin, AMX; cefepime, CEF; gentamicin, GEN; amikacin, AMK; vancomycin, VAN; and ciprofloxacin, CIP; on the production of PCs in biofilms of <i>C. albicans</i> bloodstream isolates. 48 h-mature biofilms (<i>n</i> = 6) grown in RPMI-1640 supplemented with antibiotics were treated with 100 μg ml<sup>-1</sup> amphotericin B and then evaluated for PCs. Biofilms grown in the presence of antibiotics produced more PCs, up to 10×, when exposed to AMX and CIP; 5 × to CEF; and 6 × to GEN and VAN. The results indicate that antibiotics can modulate PC production in <i>C. albicans</i> biofilms. This scenario may have clinical repercussions in immunocompromised patients under broad-spectrum antibiotic therapy.
Authors
- Bruno Rocha Amando (ORCID: https://orcid.org/0000-0003-1025-682X)
- Gláucia Morgana de Melo Guedes (ORCID: https://orcid.org/0000-0002-5469-8120)
- Pedro de Freitas Santos Manzi de Souza (ORCID: https://orcid.org/0000-0003-1893-2992)
- Ana Raquel Colares de Andrade (ORCID: https://orcid.org/0009-0007-8785-6694)
- Lívia Maria Galdino Pereira (ORCID: https://orcid.org/0000-0002-3630-9646)
- Fernando Victor Monteiro Portela (ORCID: https://orcid.org/0000-0002-6112-7748)
- Nicole de Mello Fiallos (ORCID: https://orcid.org/0000-0001-8410-1607)
- Bruno Nascimento da Silva
- Paulo Henrique Soares Peixoto (ORCID: https://orcid.org/0009-0007-8324-6194)
- Rossana de Aguiar Cordeiro (ORCID: https://orcid.org/0000-0001-8386-2180)
- Débora de Souza Collares Maia Castelo‐Branco (ORCID: https://orcid.org/0000-0002-6045-2354)
- Reginaldo Gonçalves de Lima-Neto (ORCID: https://orcid.org/0000-0002-8846-877X)
Institutions
- Universidade Federal do Ceará (BR)
- Universidade Federal de Pernambuco (BR)
- University of Florida (US)
Publication Details
- Journal
- Biofouling
- Published
- 2024-09-01
- DOI
- https://doi.org/10.1080/08927014.2024.2396013
- Primary Topic
- Antifungal resistance and susceptibility
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Conselho Nacional de Desenvolvimento Científico e Tecnológico