Transcriptomic Analysis Reveals the Heterogeneous Role of Conducting Films Upon Electrical Stimulation
Central nervous system (CNS) injuries and neurodegenerative diseases have markedly poor prognoses and can result in permanent dysfunction due to the general inability of CNS neurons to regenerate. Differentiation of transplanted stem cells has emerged as a therapeutic avenue to regenerate tissue architecture in damaged areas. Electrical stimulation is a promising approach for directing the differentiation outcomes and pattern of outgrowth of transplanted stem cells, however traditional inorganic bio-electrodes can induce adverse effects such as inflammation. This study demonstrates the implementation of two organic thin films, a polymer/reduced graphene oxide nanocomposite (P(rGO)) and PEDOT:PSS, that have favorable properties for implementation as conductive materials for electrical stimulation, as well as an inorganic indium tin oxide (ITO) conductive film. Transcriptomic analysis reveals that electrical stimulation improves neuronal differentiation of SH-SY5Y cells on all three films, with the greatest effect for P(rGO). Unique material- and electrical stimuli-mediated effects are observed, associated with differentiation, cell-substrate adhesion, and translation. The work demonstrates that P(rGO) and PEDOT:PSS are highly promising organic materials for the development of biocompatible, conductive scaffolds that will enhance electrically-aided stem cell therapeutics for CNS injuries and neurodegenerative diseases.
Authors
- Yu Suk Choi (ORCID: https://orcid.org/0000-0002-0241-0447)
- Vincent P. Wallace (ORCID: https://orcid.org/0000-0003-3814-5400)
- Per B. Zetterlund (ORCID: https://orcid.org/0000-0003-3149-4464)
- Yin Yao
- Minghao Zheng (ORCID: https://orcid.org/0000-0003-1185-4768)
- Uditi Bhatt (ORCID: https://orcid.org/0000-0003-4188-0457)
- Dino Spagnoli (ORCID: https://orcid.org/0000-0001-6367-4748)
- Cameron W. Evans (ORCID: https://orcid.org/0000-0003-2312-9803)
- Sebastian E. Amos (ORCID: https://orcid.org/0000-0003-1324-4533)
- K. Swaminathan Iyer (ORCID: https://orcid.org/0000-0001-9329-4930)
- Irene Suarez‐Martinez (ORCID: https://orcid.org/0000-0002-1877-6574)
- Alan R. Harvey
- Haibo Jiang (ORCID: https://orcid.org/0000-0002-2384-4826)
- Stuart I. Hodgetts (ORCID: https://orcid.org/0000-0002-3318-0410)
- Vipul Agarwal (ORCID: https://orcid.org/0000-0002-6239-5410)
- Nicholas B. Lawler (ORCID: https://orcid.org/0000-0002-8302-5371)
- Priya Kaluskar (ORCID: https://orcid.org/0000-0001-7414-3635)
- Kai Chen (ORCID: https://orcid.org/0000-0001-6850-1723)
Institutions
- Australian Research Council (AU)
- The University of Melbourne (AU)
- The University of Western Australia (AU)
- Curtin University (AU)
- UNSW Sydney (AU)
- Perron Institute for Neurological and Translational Science (AU)
- ARC Centre of Excellence in Advanced Molecular Imaging (AU)
- University of Hong Kong (HK)
Publication Details
- Journal
- Advanced Healthcare Materials
- Published
- 2024-09-02
- DOI
- https://doi.org/10.1002/adhm.202400364
- Citations
- 4
- Primary Topic
- Graphene and Nanomaterials Applications
- Type
- article
- Field-Weighted Citation Impact
- 0.74
Funders
- Australian Government
- Analytical Center for the Government of the Russian Federation
- University of New South Wales
- Government of Western Australia
- Medical Research Council
- Australian Research Council
- National Health and Medical Research Council