The Arthropoda-specific Tramtrack group BTB protein domains use previously unknown interface to form hexamers
BTB (bric-a-brack, Tramtrack, and broad complex) is a diverse group of protein-protein interaction domains found within metazoan proteins. Transcription factors contain a dimerizing BTB subtype with a characteristic N-terminal extension. The Tramtrack group (TTK) is a distinct type of BTB domain, which can multimerize. Single-particle cryo-EM microscopy revealed that the TTK-type BTB domains assemble into a hexameric structure consisting of three canonical BTB dimers connected through a previously uncharacterized interface. We demonstrated that the TTK-type BTB domains are found only in Arthropods and have undergone lineage-specific expansion in modern insects. The <i>Drosophila</i> genome encodes 24 transcription factors with TTK-type BTB domains, whereas only four have non-TTK-type BTB domains. Yeast two-hybrid analysis revealed that the TTK-type BTB domains have an unusually broad potential for heteromeric associations presumably through a dimer-dimer interaction interface. Thus, the TTK-type BTB domains are a structurally and functionally distinct group of protein domains specific to Arthropodan transcription factors.
Authors
- Olga Arkova (ORCID: https://orcid.org/0000-0002-5353-0748)
- А. М. Хрусталева (ORCID: https://orcid.org/0000-0003-0599-8933)
- Artem Bonchuk (ORCID: https://orcid.org/0000-0002-0948-0640)
- Rozbeh Baradaran (ORCID: https://orcid.org/0000-0002-6096-9169)
- Anna D. Burtseva (ORCID: https://orcid.org/0000-0003-0675-1043)
- Andreas Naschberger (ORCID: https://orcid.org/0000-0002-7275-5459)
- Pavel Georgiev (ORCID: https://orcid.org/0000-0002-6509-3194)
- Karina K Khalisova (ORCID: https://orcid.org/0000-0002-7384-7910)
- Vladimir O. Popov (ORCID: https://orcid.org/0000-0002-0133-7962)
- Konstantin M. Boyko (ORCID: https://orcid.org/0000-0001-8229-189X)
- Nikolai N. Sluchanko (ORCID: https://orcid.org/0000-0002-8608-1416)
- Konstantin Balagurov (ORCID: https://orcid.org/0000-0002-8650-1839)
Institutions
- Russian Academy of Sciences (RU)
- Moscow Institute of Physics and Technology (RU)
- Institute of Gene Biology (RU)
- King Abdullah University of Science and Technology (SA)
Publication Details
- Journal
- eLife
- Published
- 2024-09-02
- DOI
- https://doi.org/10.7554/elife.96832
- Citations
- 15
- Primary Topic
- Insect Resistance and Genetics
- Type
- article
- Field-Weighted Citation Impact
- 3.27
Funders
- European Synchrotron Radiation Facility
- King Abdullah University of Science and Technology
- Russian Science Foundation
- Ministry of Science and Higher Education of the Russian Federation