593. DEEP LEARNING-BASED PROGNOSTIC MODEL INTEGRATING TUMOR-INFILTRATING LYMPHOCYTES FOR ESOPHAGEAL CANCER: A MULTICENTER RETROSPECTIVE COHORT STUDY

Abstract Background Tumor-infiltrating lymphocytes (TILs) have been proven to be important prognostic factors for various tumors. However, their prognostic significance within the context of esophageal squamous cell carcinoma (ESCC) remains inadequately explored. This study aims to assess the prognostic potential of TILs in ESCC using deep learning (DL) methods. Materials and Methods We retrospectively enrolled 626 pathologically confirmed ESCC patients from two research centers. Their digital whole-slide imaging (WSI) and corresponding clinical information were collected. Subsequently, the DL method was employed to identify the tumor margin and TILs within the WSI. Tissue was divided into intra-tumor, peritumoral and stromal regions based on their distance from the tumor margin. TILs were counted in each region. Optimal cut-off values of TILs were determined using the X-tile software. To mitigate selection bias and intergroup heterogeneity, a propensity score matching (PSM) analysis was employed. Survival analysis was performed using Kaplan-Meier curves and the log-rank test. The Cox proportional hazards regression model was used to identify independent prognostic factors. Results We classified patients based on the cell counts and cut-off values of intra-tumor-infiltrating lymphocytes (I-TILs) and peritumoral infiltrating lymphocytes (P-TILs). Patients with high I-TILs & P-TILs were defined as those whose counts of both I-TILs and P-TILs exceeded the determined cut-off value. Patients with high I-TILs & P-TILs showed significantly better overall survival (OS, P = 0.0092) and recurrence-free survival (RFS, P = 0.0088) than patients with low I-TILs & P-TILs after PSM. Multivariable Cox proportional hazards regression further supported this conclusion and recognized I-TILs & P-TILs as an independent prognostic factor (P = 0.0136, hazard ratio = 0.63 for OS; P = 0.0098, hazard ratio = 0.63 for RFS). Conclusion In the present study, we identified the quantitative distribution of TILs in ESCC patients with the help of the DL method. We established that I-TILs & P-TILs serve as independent prognostic factors for these patients. Further study should focus on the lymphocyte subgroups and make better use of the spatial information to improve the predictive efficacy of TILs.