Temporal and spatial resolution of magnetosome degradation at the subcellular level in a 3D lung carcinoma model
Magnetic nanoparticles offer many exciting possibilities in biomedicine, from cell imaging to cancer treatment. One of the currently researched nanoparticles are magnetosomes, magnetite nanoparticles of high chemical purity synthesized by magnetotactic bacteria. Despite their therapeutic potential, very little is known about their degradation in human cells, and even less so of their degradation within tumours. In an effort to explore the potential of magnetosomes for cancer treatment, we have explored their degradation process in a 3D human lung carcinoma model at the subcellular level and with nanometre scale resolution. We have used state of the art hard X-ray probes (nano-XANES and nano-XRF), which allow for identification of distinct iron phases in each region of the cell. Our results reveal the progression of magnetite oxidation to maghemite within magnetosomes, and the biosynthesis of magnetite and ferrihydrite by ferritin.
Authors
- M. L. Fdez-Gubieda (ORCID: https://orcid.org/0000-0001-6076-7738)
- Ana Garcı́a-Prieto (ORCID: https://orcid.org/0000-0001-7080-3974)
- Lucía Gandarias (ORCID: https://orcid.org/0000-0001-5749-971X)
- Alicia G. Gubieda (ORCID: https://orcid.org/0000-0002-7598-2008)
- Ana Abad (ORCID: https://orcid.org/0000-0001-6022-1751)
- Ajith Pattammattel (ORCID: https://orcid.org/0000-0002-5956-7808)
- Mihály Pósfai (ORCID: https://orcid.org/0000-0001-9355-3533)
Institutions
- Centre National de la Recherche Scientifique (FR)
- University of Pannonia (HU)
- University of the Basque Country (ES)
- Brookhaven National Laboratory (US)
- Commissariat à l'Énergie Atomique et aux Énergies Alternatives (FR)
- Veszprémi Érseki Hittudományi Fõiskola (HU)
- Institut de Biosciences et Biotechnologies (FR)
Publication Details
- Journal
- Journal of Nanobiotechnology
- Published
- 2024-09-02
- DOI
- https://doi.org/10.1186/s12951-024-02788-8
- Citations
- 2
- Primary Topic
- Geomagnetism and Paleomagnetism Studies
- Type
- article
- Field-Weighted Citation Impact
- 0.47
Funders
- U.S. Department of Energy
- Ministerio de Ciencia, Innovación y Universidades
- European Synchrotron Radiation Facility
- Eusko Jaurlaritza
- Euskal Herriko Unibertsitatea
- Nemzeti Kutatási Fejlesztési és Innovációs Hivatal
- Office of Science
- European Regional Development Fund
- Agencia Estatal de Investigación
- National Research, Development and Innovation Office
- Brookhaven National Laboratory