Validation of a direct multiplex real-time reverse transcription PCR assay for rapid detection of African swine fever virus using swine field samples in Vietnam
The rRT-PCR assay demonstrated a diagnostic sensitivity of 98.8% and a specificity of 100%. Remarkably, crude samples yielded results comparable to those of purified samples, indicating the feasibility of using crude samples without compromising accuracy in ASFV detection. Our findings emphasize the effectiveness of the rRT-PCR assay for the prompt and accurate diagnosis of both swine fever viruses, which is essential for effective disease prevention and control in swine populations.
Authors
- Hiroaki Shirafuji (ORCID: https://orcid.org/0000-0002-8912-6544)
- Anh Duc Truong
- Mizuki Watanabe (ORCID: https://orcid.org/0000-0002-4030-4653)
- Hoang Vu Dang (ORCID: https://orcid.org/0000-0003-0006-7902)
- Takehiro Kokuho (ORCID: https://orcid.org/0000-0002-8869-1402)
- Ha Thi Thanh Tran (ORCID: https://orcid.org/0000-0001-7342-8815)
- Tatsuya Nishi (ORCID: https://orcid.org/0009-0003-9568-7262)
- Kentaro Masujin
- Tomoya Kitamura (ORCID: https://orcid.org/0000-0002-0251-7543)
Institutions
- National Agriculture and Food Research Organization (JP)
- National Institute of Animal Health (JP)
- National Institute of Veterinary Research (VN)
Publication Details
- Journal
- BMC Research Notes
- Published
- 2024-09-02
- DOI
- https://doi.org/10.1186/s13104-024-06898-2
- Citations
- 1
- Primary Topic
- Animal Disease Management and Epidemiology
- Type
- article
- Field-Weighted Citation Impact
- 0.57
Funders
- Ministry of Agriculture, Forestry and Fisheries