Protocol for volume correlative light X-ray and electron microscopy of endothelial cells in mouse tissue
Correlative light and electron microscopy (CLEM) greatly facilitate capturing the ultrastructure of spatially and/or temporally rare events. Here, we present a protocol for targeting regions of interests (ROIs) in tissue endothelial cells (ECs) using X-ray micro-computed tomography (μCT). We describe steps for ROI targeting guided by vasculature patterns and positions of EC nuclei visualized by light and X-ray microscopy. The protocol is applicable to thin or translucent tissues that contain defined landmarks visible in both light and X-ray microscopy. For complete details on the use and execution of this protocol, please refer to Reglero-Real et al.<sup>1</sup>.
Authors
- Azumi Yoshimura (ORCID: https://orcid.org/0000-0001-6072-6864)
- Sussan Nourshargh (ORCID: https://orcid.org/0000-0001-5677-1806)
- Lucy Collinson (ORCID: https://orcid.org/0000-0003-0260-613X)
- Rebecca S. Saleeb (ORCID: https://orcid.org/0000-0002-6587-9867)
- Natalia Reglero-Real (ORCID: https://orcid.org/0000-0002-3916-119X)
- Lorena Pérez (ORCID: https://orcid.org/0000-0001-8135-4975)
Institutions
- Queen Mary University of London (GB)
- Yamaguchi University (JP)
- The Francis Crick Institute (GB)
- CIC bioGUNE (ES)
- Centro de Biología Molecular Severo Ochoa (ES)
- William Harvey Research Institute (GB)
- Universidad Autónoma de Madrid (ES)
- University of Edinburgh (GB)
Publication Details
- Journal
- STAR Protocols
- Published
- 2024-09-01
- DOI
- https://doi.org/10.1016/j.xpro.2024.103257
- Primary Topic
- Advanced Fluorescence Microscopy Techniques
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Wellcome Trust
- Francis Crick Institute
- Cancer Research UK
- Medical Research Council
- Agencia Estatal de Investigación