A Technique for Generating Neural Progenitor Cells from Mouse Embryonic Stem Cells

Source: Mao, X., et al. Neuronal Differentiation from Mouse Embryonic Stem Cells In vitro. J. Vis. Exp. (2020). This video demonstrates a method for the in vitro generation of neural progenitor cells (NPCs) from mouse embryonic stem cells (mESCs). The mESCs are cultured in suspension to form embryoid bodies (EBs), which are then guided to differentiate into NPCs via retinoic acid treatment in an adherent culture.

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Publication Details

Journal
Journal of Visualized Experiments
Published
2024-08-30
DOI
https://doi.org/10.3791/22409
Primary Topic
Pluripotent Stem Cells Research
Type
article
Field-Weighted Citation Impact
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article

A Technique for Generating Neural Progenitor Cells from Mouse Embryonic Stem Cells

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Journal of Visualized Experiments
Pluripotent Stem Cells Research
article

A Technique for Generating Neural Progenitor Cells from Mouse Embryonic Stem Cells

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article en

Abstract

Source: Mao, X., et al. Neuronal Differentiation from Mouse Embryonic Stem Cells In vitro. J. Vis. Exp. (2020). This video demonstrates a method for the in vitro generation of neural progenitor cells (NPCs) from mouse embryonic stem cells (mESCs). The mESCs are cultured in suspension to form embryoid bodies (EBs), which are then guided to differentiate into NPCs via retinoic acid treatment in an adherent culture.

Journal of Visualized Experiments
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Pluripotent Stem Cells Research
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