Protocol for establishing and evaluating a cancer cachexia mouse model
Cancer cachexia mouse models are needed to recapitulate the clinical features of patients with cachexia. Here, we present a protocol for the establishment and evaluation of cancer cachexia mouse models. We delineate the steps in preparing tumor cells for inoculation and surgical procedures. After the establishment of these mouse models, we describe essential techniques to assess cancer cachexia, including grip strength evaluation, tissue collection, and the calculation of cross-sectional areas of muscle tissue. For complete details on the use and execution of this protocol, please refer to Liu et al.,<sup>1</sup> Yang et al.,<sup>2</sup> Shi et al.,<sup>3</sup> and Zhou et al.<sup>4</sup>.
Authors
- Jingxuan Yang (ORCID: https://orcid.org/0000-0003-3265-161X)
- Zhijun Zhou (ORCID: https://orcid.org/0000-0003-2226-2223)
- Xiuhui Shi (ORCID: https://orcid.org/0000-0002-5327-8247)
- Alex X. Arreola
- Min Li (ORCID: https://orcid.org/0000-0002-3971-9130)
- Yuqing Zhang (ORCID: https://orcid.org/0000-0002-4573-0192)
- Mingyang Liu (ORCID: https://orcid.org/0000-0003-4101-716X)
- Yang Cai (ORCID: https://orcid.org/0000-0002-0397-7971)
- Yiping Li (ORCID: https://orcid.org/0000-0003-2188-6958)
- Yu Ren (ORCID: https://orcid.org/0000-0002-8582-680X)
Institutions
- University of Oklahoma Health Sciences Center (US)
- The University of Texas Health Science Center at Houston (US)
Publication Details
- Journal
- STAR Protocols
- Published
- 2024-08-31
- DOI
- https://doi.org/10.1016/j.xpro.2024.103281
- Citations
- 7
- Primary Topic
- Muscle Physiology and Disorders
- Type
- article
- Field-Weighted Citation Impact
- 1.64
Funders
- William and Ella Owens Medical Research Foundation
- National Institutes of Health
- National Cancer Institute