PCR Detection of Spreading TEM and CTX-M gene in Klebsiella oxytoca isolate from Urinary Tract Infection

Background: urine is usually free of bacterial infection occurs by ascending or descending infection pathway. CTX-M enzymes are a group of class A extended-spectrum β-lactamases (ESBLs) that are rapidly spreading among Enterobacteriaceae, Most of the ESBLs are mutants of the TEM and the SHV enzymes and developed from point mutation of genes that code production of primordial TEM-1, TEM-2, or SHV-1 β lactamase enzymes. Objectives: This study aimed to detect K. oxytoca bacteria in urine samples obtained from individuals with urinary tract infections.and, we aimed to the existence of blaCTX-M and blaTEM genes in Klebsiella oxytoca in Najaf governorate from November 2022 to June 2023. Methodology: The study included 540 urine specimens. Based on culture and biochemical features, K. oxytoca isolates were identified. The Vitek-2 compact system provides a confirmatory test, PCR was used for detecting the blaCTX-M and blaTEM genes. Result:seventeen isolates were identified as K. oxytoca. Vitek further confirmed this, PCR technique targeting the blaCTX-M gene detected in 14 isolates, while 4 from the 17 isolates were positive for the blaTEM gene.Conclusion: The molecular study revealed that blaCTX-M and blaTEM gene were detected in (82.3%) and (23.5%) of K. oxytoca isolates carrying blaCTX-M and blaTEM genes respectively.