Detection of Parasites in Locally Sourced Fresh Fruits and Vegetables Using Various Diagnostic Techniques

A prospective study was conducted from September 2022 to March 2023 to examine the occurrence and contamination rates of different parasitic stages on fresh edible fruits and vegetables in local markets of Erbil city. Eight different types of fruits and vegetables were selected from eight markets, and 144 samples were analyzed for parasites using direct sedimentation, indirect floatation (ZnSO4), Kinyoun, and trichrome staining methods. The highest total frequencies of parasites were detected using the direct sedimentation method (18.75%), followed by the indirect floatation method (6.25%), Kinyoun technique (5.55%), and modified trichrome staining (4.16%). A. lumbricoides (Ova) was detected with 4.16% using direct sedimentation, while no positive detections were observed with other techniques. Kinyoun technique identified Cryptosporidium spp. oocysts. For E. histolytica 1.3% were positive using the direct sedimentation method, while the other three methods showed no positive detections. Similar results were observed for G. lamblia where only the modified trichrome staining technique detected 0.69% with trophozoites and 1.3% with cysts. Additionally, the direct sedimentation method revealed the presence of nematodes at 2.77%, but no positive detections were made with other methods. Concerning Taeniid eggs, the indirect floatation identified 0.69% with ova, while other methods showed no positive detections. Lastly, the direct sedimentation method yielded 6.25% with unidentified flagellate protozoa, but no positive detections were found using the indirect floatation method, Kinyoun technique, or modified trichrome staining technique. The study concluded that Kinyoun and trichrome techniques are the gold standard for detecting different parasite stages.