Duck enteritis virus LORF4 gene is a late gene and nonessential for virus replication in vitro

Duck enteritis virus (DEV) is an avian alpha-herpesvirus that primarily causes an acute and highly contagious infectious disease of ducks. The LORF4 gene is one of the specific genes of DEV, with limited reports on its biological characteristics and functions. This study investigates the basic biological properties of LORF4 protein (pLORF4). The results show that DEV LORF4 is a late gene mainly localized in the cytoplasm of DEV-infected DEF. To explore the role of pLORF4 in the DEV replication life cycle, a recombinant virus lacking pLORF4 expression was constructed. The results showed that pLORF4 is not essential for virus replication and does not affect virus adsorption, assembly and release, it plays a positive role in virus invasion and DNA replication. In summary, this study provides a foundation for further research on the function of the LORF4 gene.

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Publication Details

Journal
Poultry Science
Published
2024-08-31
DOI
https://doi.org/10.1016/j.psj.2024.104275
Primary Topic
Herpesvirus Infections and Treatments
Type
article
Field-Weighted Citation Impact
0.00

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article

Duck enteritis virus LORF4 gene is a late gene and nonessential for virus replication in vitro

Mafeng Liu, Ying Wu, Jie Huang, Shaqiu Zhang et al.
Poultry Science
Herpesvirus Infections and Treatments
article

Duck enteritis virus LORF4 gene is a late gene and nonessential for virus replication in vitro

Mafeng Liu, Ying Wu, Jie Huang, Shaqiu Zhang, Anchun Cheng, Renyong Jia, Dekang Zhu, Zhen Wu, Juan Huang, Shun Chen, Bin Tian, Mingshu Wang, Yu He, Qiao Yang, Di Sun, Xumin Ou, Xinxin Zhao
article en

Abstract

Duck enteritis virus (DEV) is an avian alpha-herpesvirus that primarily causes an acute and highly contagious infectious disease of ducks. The LORF4 gene is one of the specific genes of DEV, with limited reports on its biological characteristics and functions. This study investigates the basic biological properties of LORF4 protein (pLORF4). The results show that DEV LORF4 is a late gene mainly localized in the cytoplasm of DEV-infected DEF. To explore the role of pLORF4 in the DEV replication life cycle, a recombinant virus lacking pLORF4 expression was constructed. The results showed that pLORF4 is not essential for virus replication and does not affect virus adsorption, assembly and release, it plays a positive role in virus invasion and DNA replication. In summary, this study provides a foundation for further research on the function of the LORF4 gene.

Poultry ScienceVol. 103(12)
Ministry of Education of the People's Republic of China (CN), Sichuan Agricultural University (CN), Sichuan Center for Disease Control and Prevention (CN)
Agriculture Research System of China, Ministry of Agriculture and Rural Affairs of the People's Republic of China, China Agricultural Research System, Sichuan Veterinary Medicine and Drug Innovation Group of China Agricultural Research System
Zero hunger
Openalex Percentile: Top 78%
Herpesvirus Infections and Treatments
DOIOpen Access PDFOpenAlex
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