Engineered IRES-mediated promoter-free insulin-producing cells reverse hyperglycemia
In summary, our study demonstrates that Cytopore I microcarriers are biocompatible and promote long-term cell survival <i>in vivo</i>. The promoter-free EMCVIRES-insulin loop enables non-endocrine cells to secrete mature insulin, leading to a rapid reduction in glucose levels. We have presented a novel promoter-free genetic engineering strategy for insulin secretion and proposed an efficient cell transplantation method. Our findings suggest the potential to expand the range of cell sources available for the treatment of diabetes, offering new avenues for therapeutic interventions.
Authors
- Chengming Ni
- Yunting Zhou (ORCID: https://orcid.org/0000-0002-1385-3841)
- Cong He (ORCID: https://orcid.org/0000-0002-1185-5456)
- Rui Liu (ORCID: https://orcid.org/0000-0002-0549-3532)
- Yumin Li (ORCID: https://orcid.org/0000-0002-3557-5969)
- Zilin Sun (ORCID: https://orcid.org/0000-0003-1865-1429)
- Bo Sun (ORCID: https://orcid.org/0000-0002-1660-2378)
- Hao Lin (ORCID: https://orcid.org/0000-0001-7981-7707)
- Zhongdang Xiao (ORCID: https://orcid.org/0000-0003-2288-951X)
- Doulathunnisa Ahamed Younis
Institutions
- University of Suwon (KR)
- Jiangsu Second Normal University (CN)
- Zhongda Hospital Southeast University (CN)
- State Key Laboratory of Digital Medical Engineering (CN)
- UConn Health (US)
- Southeast University (CN)
- Nanjing Medical University (CN)
Publication Details
- Journal
- Frontiers in Endocrinology
- Published
- 2024-08-30
- DOI
- https://doi.org/10.3389/fendo.2024.1439351
- Citations
- 2
- Primary Topic
- Pancreatic function and diabetes
- Type
- article
- Field-Weighted Citation Impact
- 1.15
Funders
- National Natural Science Foundation of China
- Government of Jiangsu Province
- Fundamental Research Funds for the Central Universities