Development and evaluation of a rapid visual loop-mediated isothermal amplification assay for the <i>tcdA</i> gene in <i>Clostridioides difficile</i> detection
LAMP method is an effective technique for the rapid and visual detection of the <i>tcdA</i> gene of <i>C. difficile</i>, and shows potential advantages over PCR in terms of speed, simplicity, and sensitivity. The <i>tcdA</i>-LAMP assay is particularly suitable for medical diagnostic environments with limited resources and is a promising diagnostic strategy for the screening and detection of <i>C. difficile</i> infection in populations at high risk.
Authors
- Jiasheng Tan
- Bingyun Lu (ORCID: https://orcid.org/0000-0002-0358-1379)
- Ming Jin
- Jing Yuan (ORCID: https://orcid.org/0000-0002-6939-9676)
- Minyi Lin
- Pu Wang (ORCID: https://orcid.org/0000-0002-8121-1568)
- Wei Liu (ORCID: https://orcid.org/0000-0003-4955-5720)
- Xiao-Mou Peng
- Ye Chen (ORCID: https://orcid.org/0009-0001-0503-5777)
Institutions
- Sun Yat-sen University (CN)
- Academy of Military Medical Sciences (CN)
- Nanfang Hospital (CN)
- Fifth Affiliated Hospital of Sun Yat-sen University (CN)
- Songshan Lake Materials Laboratory (CN)
- Southern Medical University Shenzhen Hospital (CN)
- Southern Medical University (CN)
Publication Details
- Journal
- PeerJ
- Published
- 2024-08-30
- DOI
- https://doi.org/10.7717/peerj.17776
- Citations
- 2
- Primary Topic
- Clostridium difficile and Clostridium perfringens research
- Type
- article
- Field-Weighted Citation Impact
- 0.78
Funders
- National Natural Science Foundation of China
- Basic and Applied Basic Research Foundation of Guangdong Province