Detection of anti-bacterial activity of silver nanoparticles from actinomycetes and testing its efficacy

Silver nanoparticles rank among the most promising metal nanoparticles that are less than 100 nm in diameter. Their compact dimensions and substantial surface area-to-volume ratio lend them distinct physical, chemical, and biological properties that make them appealing for a multitude of applications. They can be synthesized in a variety of physical, chemical, and biological methods, and one such technique is the green route synthesis of AgNPs from actinomycetes. While AgNPs have immense potential in a diverse range of applications, its bactericidal effect is the focus of this current work. AgNPs' antibacterial activity was evaluated in both gram-positive and gram-negative bacteria at concentrations ranging from 25 to 150 μg. The bactericidal effect was more pronounced in gram-negative bacteria when compared to gram-positive bacteria. They exhibit twin actions of bactericidal assault and membrane rupture, which enable them to induce cell lysis and interfere with bacterial protein synthesis. Often alluded to as "Nano-antibiotics," these compounds may also serve as delivery systems for conventional antibiotics.