Hydrophobic Paper Used to Construct a Disposable Chemiresistive Immunosensor for the Simultaneous Detection of FB1 and AFB1

Abstract Co‐contamination of mycotoxins produced by fungi in foodstuffs and feeds causes a tremendous health risk to humans and animals. The simultaneous detection of multiple mycotoxins using a cost‐effective and point‐of‐care technology is key to ensuring food safety. In this study, a disposable biosensor with two individual sensing channels is prepared on an affordable cellulose paper substrate and used to simultaneously detect fumatoxin B1 (FB1) and aflatoxin B1 (AFB1). The biosensor pattern is outlined by polydimethylsiloxane (PDMS) and the hydrophobic interface of the sensing channels is created using octadecyltrichlorosilane (OTS). Semiconducting single‐wall carbon nanotubes (s‐SWCNTs) are deposited onto the central zones to serve as the sensing elements and SWCNTs are deposited into the bilateral areas as wire. After functionalization of the s‐SWCNTs, anti‐FB1 and anti‐AFB1 are separately immobilized on the different sensing regions to capture the targeted mycotoxins. Under the optimal conditions, this developed s‐SWCNTs‐based biosensor array achieved a limit of detection (LOD) of 8.23 pg mL −1 for FB1 and 7.48 pg mL −1 for AFB1. As a demonstration, spiked corn samples are measured using this biosensor and recovery rates are not inferior to commercial enzyme‐linked immune sorbent assay (ELISA) kits. Overall, the cost‐effective, highly sensitive, and multiplexed biosensor platform fabricated by this approach shows great potential for detecting multiple mycotoxins.