Multiomics analysis reveals the potential mechanism of high‐fat diet in dextran sulfate sodium‐induced colitis mice model

Abstract A high‐fat diet (HFD) is recognized as an important contributor to inflammatory bowel disease (IBD). However, the precise underlying mechanism of HFD on IBD remains elusive. This study aimed to investigate the potential mechanism by which HFD affects IBD using 16S rRNA‐sequencing and RNA‐seq technology. Results indicated that HFD‐treated mice exhibited notable alternations in the structure and composition of the gut microbiota, with some of these alternations being associated with the pathogenesis of IBD. Analysis of the colon transcriptome revealed 11 hub genes and 7 hub pathways among control, DSS‐induced colitis, and HFD + DSS‐treated groups. Further analysis explores the relationship between the hub pathways and genes, as well as the hub genes and gut microbiota. Overall, the findings indicate that the impact of HFD on DSS‐induced colitis may be linked to intestinal dysbiosis and specific genes such as Abca8b , Ace2 , Apoa1 , Apoa4 , Apoc3 , Aspa , Dpp4 , Maob , Slc34a2 , Slc7a9 , and Trpm6 . These results provide valuable insights for determining potential therapeutic targets for addressing HFD‐induced IBD.