Influência do processo extrativo na atividade antimicrobiana de Croton antisyphiliticus Mart. (Euphorbiaceae)

The present study aimed to evaluate the influence of the factors drug / solvent ratio (p/v) and time (days) in the maceration extraction process of Croton antisyphiliticus Mart. on antimicrobial activity and polyphenolic compound content. The extracts were obtained by maceration of the aerial parts, using a factorial design 32, considering 3 proportions 1:10, 1:20 and 1:30; and 7, 10 and 13 days. Extracts were characterized in total solids content, total polyphenols content and gallic acid. Antimicrobial activity against Staphylococcus aureusand Escherichia coli was evaluated using the microdilution technique. Only the minimum inhibitory concentration (MIC) was shown to be influenced by the factors chosen, so that the higher the drug / solvent ratio and the longer the extraction time, the better the MIC values. The F3 experiment (1:30 p/v and 13 days) obtained the best results in relation to the content of total polyphenols, gallic acid and MIC, with values ​​equal to 411.37 mg GAE / g, 75.97 mg GAE / g and 0.001 mg / mL, respectively.