Broad range molecular detection methods identify only Borrelia spp. in erythema migrans biopsies and blood of tick-bitten patients

In this multicenter study conducted in France, we challenged the hypothesis of the transmission of pathogens other than <i>Borrelia</i> spp. in 22 patients developing erythema migrans following a tick bite. Using a combination of high-throughput microfluidic PCRs and agnostic metagenomics on skin biopsies and blood samples, no microorganisms other than <i>Borrelia</i> spp. was found.

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Publication Details

Journal
One Health
Published
2024-08-30
DOI
https://doi.org/10.1016/j.onehlt.2024.100886
Primary Topic
Vector-borne infectious diseases
Type
article
Field-Weighted Citation Impact
0.00

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article

Broad range molecular detection methods identify only Borrelia spp. in erythema migrans biopsies and blood of tick-bitten patients

Élisabeth Botelho-Nevers, Pascale Frey-Klett, Nicole Corre-Catelin, Philippe Pérot et al.
One Health
Vector-borne infectious diseases
article

Broad range molecular detection methods identify only Borrelia spp. in erythema migrans biopsies and blood of tick-bitten patients

Élisabeth Botelho-Nevers, Pascale Frey-Klett, Nicole Corre-Catelin, Philippe Pérot, Costanza Puppo, Sara Moutailler, Véronique Perronne, Clémence Galon, Valentine Piquard, Arnaud Fontanet, Marie‐Noëlle Ungeheuer, Laurence Arowas, Karine Lacombe, Marc Éloit, Delphine Chrétien, Laura Tondeur, Marie Préau, Ayla Zayoud, Catherine Chirouze, Céline Cazorla, Muriel Vayssier‐Taussat
article en

Abstract

In this multicenter study conducted in France, we challenged the hypothesis of the transmission of pathogens other than <i>Borrelia</i> spp. in 22 patients developing erythema migrans following a tick bite. Using a combination of high-throughput microfluidic PCRs and agnostic metagenomics on skin biopsies and blood samples, no microorganisms other than <i>Borrelia</i> spp. was found.

One HealthVol. 19
Centre National de la Recherche Scientifique (FR), Institut Pasteur (FR), École Nationale Vétérinaire d'Alfort (FR), Université Paris Cité (FR), Centre Hospitalier Universitaire de Besançon (FR), Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement (FR), Laboratoire Chrono-Environnement (FR), Département Santé Animale (FR), Université Bourgogne Franche-Comté (FR), Bipar (FR), Agence Nationale de Sécurité Sanitaire de l’Alimentation, de l’Environnement et du Travail (FR), Centre Hospitalier Universitaire de Saint-Étienne (FR)
Agence Nationale de la Recherche
Zero hunger
Openalex Percentile: Top 87%
Vector-borne infectious diseases
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