In vitro evaluation of the anthelmintic potential of proteases from garlic peel (Allium sativum)

The garlic peel (Allium sativum) is rich in protease-type enzymes, which have already demonstrated their anthelmintic activity. The objective of the present study was to evaluate the potential use of proteases from garlic peel in the in vitro control of Moniezia expansa eggs and trichostrongylid. The prepared extract was precipitated and dialyzed, and its proteolytic activity was evaluated. Subsequently, three experimental groups were prepared: one containing the active extract, another with the denatured extract, and a control group. In vitro assays were then conducted to assess the effect of the extract on M. expansa eggs and trichostrongylid (Haemonchus spp. and Trichostrongylus spp.). There was an increase in enzymatic activity from 4.39 U/mL to 7.32 U/mL after precipitation and dialysis. In vitro experiments confirmed the nematicidal and ovicidal efficacy of garlic peel proteases, with an average reduction of L3 in the experimental groups, in comparison with the control, were: 9,16 % for the denatured extract and 55% for the active extract and 27.69% for M. expansa eggs. This is the first report of the nematicidal and ovicidal effect of these parasites using enzymes from garlic peel. Further research should explore the potential of garlic waste enzymes to create products that contribute to parasite control.