Protocol for detecting nitrative stress in biological lipid membranes in murine cells and tissues
Detection of nitrative stress is crucial to understanding redox signaling and pathophysiology. Dysregulated nitrative stress, which generates high levels of peroxynitrite, can damage lipid membranes and cause activation of proinflammatory pathways associated with pulmonary complications. Here, we present a protocol for implementing a peroxynitrite-sensing phospholipid to investigate nitrative stress in murine cells and lung tissue. We detail procedures for sensing ONOO<sup>-</sup> in stimulated cells, both ex vivo and in vivo, using murine models of acute lung injury (ALI). For complete details on the use and execution of this protocol, please refer to Gutierrez and Aggarwal et al.<sup>1</sup>.
Authors
- Andrew J. Gow (ORCID: https://orcid.org/0000-0003-0876-5158)
- Emily Stevenson (ORCID: https://orcid.org/0000-0003-1329-8734)
- J J Herbert (ORCID: https://orcid.org/0000-0001-5676-2512)
- Tushar Aggarwal (ORCID: https://orcid.org/0000-0002-6641-0964)
- Alyssa Bellomo
- Debra L. Laskin (ORCID: https://orcid.org/0000-0003-1832-7311)
- Enver Çagrı Izgü (ORCID: https://orcid.org/0000-0001-6673-3635)
Institutions
- Rutgers, The State University of New Jersey (US)
Publication Details
- Journal
- STAR Protocols
- Published
- 2024-08-30
- DOI
- https://doi.org/10.1016/j.xpro.2024.103268
- Citations
- 1
- Primary Topic
- Analytical Chemistry and Sensors
- Type
- article
- Field-Weighted Citation Impact
- 0.29
Funders
- American Cancer Society
- Rutgers Cancer Institute of New Jersey
- Rutgers, The State University of New Jersey
- National Institutes of Health
- Research Council, Rutgers, The State University of New Jersey
- Center for Environmental Health Sciences, Massachusetts Institute of Technology
- National Heart, Lung, and Blood Institute
- National Cancer Institute
- National Institute of Environmental Health Sciences
- National Institute of Arthritis and Musculoskeletal and Skin Diseases
- National Institute of Biomedical Imaging and Bioengineering